CLIA Regulations for New Test Methods

Laboratory technologist validating a new diagnostic instrument

Last updated: 2025

When a Test Is Considered New

A test method is considered new when the laboratory adds a new assay, changes platforms, significantly modifies an existing method, or implements a laboratory-developed test (LDT). CLIA requires evidence that the method performs as intended before reporting patient results.

Verification vs Validation Under CLIA

  • Verification: Confirming that a commercially cleared method performs in your lab as the manufacturer claims.
  • Validation: Establishing performance characteristics for methods not previously cleared, such as LDTs or significantly modified tests.

Required Performance Characteristics

Depending on the test type, performance studies may include:

  • Accuracy (comparison to a reference method or peer instrument).
  • Precision (within-run and between-run reproducibility).
  • Analytical measurement range and linearity.
  • Reference intervals and reportable ranges.
  • Analytical sensitivity and specificity.
  • Carryover and interference studies.

Documentation Expectations

For each new method, CLIA surveyors expect to see:

  • Written validation/verification plan and protocol.
  • Raw data and summary analysis.
  • Approval by the laboratory director or designee.
  • Updated procedure manual reflecting the new method.

How mylabcompliance.io Streamlines New Method Onboarding

mylabcompliance.io simplifies the onboarding of new methods by:

  • Providing standardized templates for validation and verification studies.
  • Capturing data, approvals, and attachments in one record per method.
  • Linking test methods to instruments, reagents, and QC requirements.
  • Tracking status of ongoing validations across multiple sites.

Related Validation Topics